Monoclonal Anti-Granulocyte Colony Stimulating Factor antibody produced in rat G5421 from MilliporeSigma

Product Specs
ItemMonoclonal Anti-Granulocyte Colony Stimulating Factor antibody produced in rat
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberG5421
Conjugate/Tagunconjugated
Reactivitymouse
TargetCsf3
IsotypeIgG1
Clone67604
Hostrat
NCBI Full Gene Namecolony stimulating factor 3 (granulocyte)
NCBI Gene AliasesCsfg, G-CSF, MGI-IG
Clonalitymonoclonal
Quantity500µG

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) Cytochrome P450 1B1 Expression Regulates Intracellular Iron Levels and Oxidative Stress in the Retinal EndotheliumInternational Journal of Molecular SciencesJanuary 26, 2023Yong-Seok Song, Ismail S. Zaitoun, Shoujian Wang, Soesiawati R. Darjatmoko, Christine M. Sorenson, Nader Sheibanifurther incubated with Erastin for another 24 h. The viability of the cells was assessed by MTS assay (G5421; Promega, Madison, WI, USA) by measuring absorbance at 490 nm using a plate reader (Epoch, BioTek Instruments,

(2) Fingolimod (FTY720), a Sphinogosine-1-Phosphate Receptor Agonist, Mitigates Choroidal Endothelial Proangiogenic Properties and Choroidal NeovascularizationCellsMarch 11, 2022Christine M. Sorenson, Mitra Farnoodian, Shoujian Wang, Yong-Seok Song, Soesiawati R. Darjatmoko, Arthur S. Polans, Nader Sheibanifor an additional 1–4 days. A cell titer 96 aqueous non-radioactive cell proliferation assay reagent (G5421; Promega, Madison, WI, USA) was added each day to a portion of the cells according to the manufacturer’s

(3) Hypoxic preconditioning induces epigenetic changes and modifies swine mesenchymal stem cell angiogenesis and senescence in experimental atherosclerotic renal artery stenosisStem Cell Research & TherapyDecember 1, 2021Busra Isik et al.a CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (Promega, Madison, WI, USA, Cat. #G5421) according to the manufacturer’s instructions. MSC migration was tested using a QCM™ Colorimetric Cell Assay

(4) Mutant-selective degradation by BRAF-targeting PROTACsNature CommunicationsDecember 1, 2021Shanique Alabi, Saul Jaime-Figueroa, Zhan Yao, Yijun Gao, John Hines, Kusal T. G. Samarasinghe, Lea Vogt, Neal Rosen, Craig M. Crewscompound for the indicated lengths of time (between 72 and 96 h). 2 mg/ml MTS (Promega Corp., Madison, WI: G5421) and 25 μM phenazine methosulfate (Sigma, St. Louis, MO) were combined 19:1 and then added to cells

(5) Targeting stanniocalcin-1-expressing tumor cells elicits efficient antitumor effects in a mouse model of human lung cancerCancer medicineMay 1, 2021Abe K, Kanehira M, Ohkouchi S, Kumata S, Suzuki Y, Oishi H, Noda M, Sakurada A, Miyauchi E, Fujiwara T, et al.arrest of cell growth was evaluated using CellTiter 96 AQueous Non‐Radioactive Cell Proliferation Assay (G5421) (Promega Corp.) according to the manufacturer's instructions. Experiments were repeated independently

(6) Comparative analysis of adeno-associated virus serotypes for gene transfer in organotypic heart slicesJournal of Translational MedicineDecember 1, 2020Zihou Liu, Kristin Klose, Sebastian Neuber, Meng Jiang, Manfred Gossen, Christof Stammtracing in ImageJ following an established protocol [33]. For the MTS assay, MTS (Promega, catalog no. G5421) and the electron coupling reagent phenazine methosulfate (Sigma, catalog no. P9625) were mixed in a

(7) KRAS Controls Pancreatic Cancer Cell Lipid Metabolism and Invasive Potential through the Lipase HSLCancer researchNovember 15, 2020Rozeveld CN, Johnson KM, Zhang L, Razidlo GL.proliferation and viability assays, the CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay (Promega, G5421) or propidium iodide (P4864, Sigma) were used. Fluorescence and live-cell microscopy Cells were washed

(8) Combinatory Treatment of Canavanine and Arginine Deprivation Efficiently Targets Human Glioblastoma Cells via Pleiotropic MechanismsCellsSeptember 30, 2020Olena Karatsai, Pavel Shliaha, Ole N. Jensen, Oleh Stasyk, Maria Jolanta Rędowiczand stored at 4 °C. The MTS reagent (CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay, G5421) was from Promega Corporation (Madison, WI, USA). 13C6,15N4-arginine was obtained from ThermoFisher Scientific

(9) Synthesis and Evaluation of Hydrogen Peroxide Sensitive Prodrugs of Methotrexate and Aminopterin for the Treatment of Rheumatoid ArthritisJournal of Medicinal ChemistryApril 26, 2018Jorge Peiró Cadahía, Jon Bondebjerg, Christian A. Hansen, Viola Previtali, Anders E. Hansen, Thomas L. Andresen, Mads H. ClausenCellTiter 96 ® AQueous Non-Radioactive Cell Proliferation Assay was purchased from Promega (MTS, G5421). Cell Culture. The human breast cancer MCF-7 (Sigma) and human large cell lung cancer NCIH460 (ATTC)

(10) Mito-Apocynin Prevents Mitochondrial Dysfunction, Microglial Activation, Oxidative Damage, and Progressive Neurodegeneration in MitoPark Transgenic MiceAntioxidants & Redox SignalingNovember 10, 2017Monica Langley et al.hydrogen peroxide (H325) were all purchased from Sigma (St. Louis, MO). The CellTiter-Glo Cell Viability assay kit (G5421) was bought from Promega (Madison, WI). We purchased RPMI 1640 (11875093), Dulbecco’s modified Eagle’s

(11) BRCA1 regulation on β-hCG: a mechanism for tumorigenicity in BRCA1 defective breast cancerOncogenesisSeptember 4, 2017S K Sengodan et al.(S4317, Sigma-Aldrich) for 48 h and the cell viability was assessed as per manufacturer’s protocol (G5421, Promega). Statistical analysis Statistical analysis was performed by one way ANOVA (Student's t-test).

(12) Extracellular serglycin upregulates the CD44 receptor in an autocrine manner to maintain self-renewal in nasopharyngeal carcinoma cells by reciprocally activating the MAPK/β-catenin axisCell death & diseaseNovember 3, 2016Chu Q, Huang H, Huang T, Cao L, Peng L, Shi S, Zheng L, Xu L, Zhang S, Huang J, et al.concentration gradient. Cell viability was measured 48 h later by MTS method (Promega, Madison, WI, USA, G5421). For cell proliferation assays, cells were seeded into 96-well plates at 1000 cells per well and treated

(13) Translational control of PML contributes to TNFα-induced apoptosis of MCF7 breast cancer cells and decreased angiogenesis in HUVECsCell Death & DifferentiationMarch 1, 2016K-S Hsu, B-J Guan, X Cheng, D Guan, M Lam, M Hatzoglou, H-Y KaoMadison, WI, USA). Cells that were <6 passages were used in this study. TNFα was purchased from Promega (G5421, Madison, WI, USA). The kinase inhibitors used in this study are: p38 kinase (SB202190; Sigma), CKII

(14) Cytotoxicity of 3-(3,5-dichlorophenyl)-2,4-thiazolidinedione (DCPT) and analogues in wild type and CYP3A4 stably transfected HepG2 cellsToxicology in VitroDecember 1, 2011Douglas M. Frederick, Erina Y. Jacinto, Niti N. Patel, Thomas H. Rushmore, Ruy Tchao, Peter J. HarvisonChemical Co. (St. Louis, MO). The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay kit (No. G5421) is a product of Promega Corp. (Madison, WI). Gibco® high glucose Dulbecco's modified Eagle's medium

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Monoclonal Anti-Granulocyte Colony Stimulating Factor antibody produced in rat from MilliporeSigma

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Monoclonal Anti-Granulocyte Colony Stimulating Factor antibody produced in rat from MilliporeSigma

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